Negative Pressure Wound Therapy (NPWT) has been introduced into acute paediatric burn care. This presentation summaries a PhD thesis aiming to investigate NPWT variables and identify how they may be optimised to help facilitate implementation and formulate a set of recommendations regarding NPWT application in acute paediatric burn care. Methodology These data for the Thesis were collected from Queensland Children’s Hospital. Various research styles were used to address the thesis aim including two randomised control trials, an ex vivo experimental model, a retrospective cohort study and a case report. Results A retrospective cohort study identified the best cohort for NPWT application was deep partial thickness burns, reducing scar clinic referral probability from 67.2% (95% CI 58.8 – 75.6) to 44.9% (95% CI 35.6 – 54.3%; p<0.001). A ex vivo model demonstrated that pressure changes were observed to the depth of the subcutis only, increasing dressing layers beneath NPWT or using circumferential NPWT dressings decreases the overall delivered pressure. A case report identified that NPWT applied without Mepitel® as a barrier between the skin and dressing may result in hypertrophic scar formation. A PICO™ randomised control trial (RCT) found that PICO™ should be used with ActicoatFlex™ as Acticoat™ had significantly more complications when combined with a PICO™ dressing (p=<0.001, mean difference 62.5%, 95% CI 29.9% - 95.1%). A hand and foot RCT found that there was no difference in dressing burden between NPWT and standard dressings and that overall clinician opinion regarding NPWT improved over the course of the study. 93% of participants reported that they would use NPWT on the hand and/or foot again. Conclusion This thesis through a collation of papers investigates NPWT variables in acute paediatric burn care. A set of recommendations for NPWT application in paediatric burn care has been developed based on these data.