Through time, humans have conveyed understanding through art. From symbolic cave drawings, abstract medieval imagery, to realism and humanism during the Renaissance. As art evolved so has the knowledge of anatomy. Early anatomical knowledge is evident in embalming practices dating back to Ancient Egypt. Herophulis (200BC), is credited as the first person to conduct human dissections, but in the Middle Ages human dissection remained taboo. It was not until the 12th century when rare Papal approval was given to universities for cadaveric dissections to further anatomical teaching. The study of anatomy has always been intrinsically tied to visual representation. As an inherently visual discipline, anatomy relies on illustration to communicate form and function. Renaissance anatomical illustrations are attributed Leonardo Da Vinci and Michelangelo. These artists, alongside anatomists such as Andreas Vesalius, produced detailed depictions of the human body that advanced anatomical understanding. Despite the increased precision, these illustrations remained confined to two-dimensional space and were limited in their ability to convey the spatial complexity and relationships of anatomical structures. Moveable or pop-up books is a medium that overcomes the challenge of two-dimensional depictions. The first moveable book is attributed to a poet of the 12th century Ramon Llull who used revolving discs to illustrate his philosophical theories. By the 16th century, anatomist adopted similar techniques for educational purposes. These include works by Andreas Vesalius and Johann Remmelin, both utilised layered, liftable images to enable sequential, three-dimensional exploration of the human body. These paper-based innovations laid the foundation for increasingly dynamic modes of anatomical visualisation. This paper will explore the history of the art of illustration, alongside the development of anatomy and how the two have intersected to give rise to pop-up anatomy books.