Introduction Traditional surgical approaches for vaginal prolapse repairs often face challenges such as recurrence and complications related to the use of synthetic mesh including erosion, pain and infection. The aim of this study was to assess the effectiveness of a novel technique for vaginal prolapse repair utilising autologous vaginal mucosal grafts. Methods This is a single-centre, single operator, retrospective study between 2017 and 2024. Patients included in the study had symptomatic vaginal prolapse and underwent repair using a novel technique of transvaginal wall repair using autologous vaginal mucosal grafts. The primary outcome measure of success was freedom from repeat procedures for prolapse and secondary measures were complications, functional outcomes and quality of life measures. Results 97 patients underwent prolapse repair (74 anterior repair only, 2 posterior repair only and 21 both anterior and posterior repair). Mean age at operation was 68. 26.8% of patients had undergone previous prolapse surgeries. Median vaginal parity was 2 (1-3). Median follow-up was 54 months (37.5-74). Freedom from repeat procedures was 93.8%. Patient reported post-operative success was 84%. There was no reported impact on quality of life related to their prolapse after surgery except for moderate use of incontinence pads. Bleeding was a reported complication in 4.1% of procedures. De novo dyspareunia was reported in 4.1% of patients, with no de novo urinary incontinence or pain. Conclusions The use of autologous vaginal mucosal grafts for vaginal prolapse repair offers a promising alternative to traditional methods, with low complication rates and lower recurrence rates without risks posed by synthetic mesh repairs.